Control of Plutella xylostella by Spodoptera exigua nuclear polyhedrosis virus in field traial.

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Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis virus.

The nucleotide sequence of a 1.1 kbp fragment of the multiple nucleocapsid nuclear polyhedrosis virus (MNPV) of Spodoptera exigua (Se) containing the polyhedrin gene was determined. An open reading frame (ORF) of 738 nucleotides (nt) was detected. This ORF encoded a protein of 246 amino acids with a predicted M(r) of 29K. The nucleotide and amino acid sequences were compared with the sequences ...

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Nucleotide sequence and transcriptional analysis of the p10 gene of Spodoptera exigua nuclear polyhedrosis virus.

The p10 gene of Spodoptera exigua multiple nuclear polyhedrosis virus (SeMNPV) was localized on the XbaI fragment H (5.1 kb) of the physical map of the viral genome. The coding sequence of the SeMNPV p10 gene is 264 nucleotides (nt) long corresponding to a predicted protein of 88 amino acids with an MHF of 9607. The SeMNPV p10 protein showed only limited amino acid identity (39% and 26%, respec...

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Activity and Persistence of Steinernema carpocapsae and Spodoptera exigua Nuclear Polyhedrosis Virus against S. exigua Larvae on Soybean.

Experiments were conducted to assess the effects of the entomopathogenic nematode Steinernema carpocapsae and Spodoptera exigua multinucleocapsid nuclear polyhedrosis virus (SeMNPV), alone and in combinations, on mortality of the beet armyworm, S. exigua, larvae on soybean. In 1991 tests, field-grown soybean plants were treated with S. carpocapsae at 0.3 and 0.6 nematodes/cm(2) of leaflet, SeMN...

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Physical Map of the DNA of a Mamestra brassicae Nuclear Polyhedrosis Virus Variant Isolated from Spodoptera exigua

The D N A of a multiply-enveloped nuclear polyhedrosis virus (MNPV) of the cabbage moth Mamestra brassicae (Mb), isolated from the beet armyworm Spodoptera exigua, was analysed using restriction endonucleases SmaI, BamHI, BgllI and PstI. The size of the viral D N A was calculated to be about 152 kilobase pairs. Mapping of restriction sites on the D N A was accomplished by hybridization of 32 P-...

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In vitro transactivation of baculovirus early genes by nuclear extracts from Autographa californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells.

Nuclear extracts, prepared from Autographa californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells during a time course of infection, were analyzed for activation of early gene transcription and for late gene transcription. The templates used in the in vitro transcription assays contained promoters for baculovirus genes that have been classified as immediate early, delayed e...

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ژورنال

عنوان ژورنال: Annual Report of The Kansai Plant Protection Society

سال: 1997

ISSN: 0387-1002

DOI: 10.4165/kapps1958.39.0_13